This DSIP dosage chart separates two sets of numbers that most dosing pages blend together. The published human trials gave DSIP (delta sleep-inducing peptide) intravenously, at about 21 to 25 micrograms per kilogram of body weight, which is roughly 1,500 to 1,800 mcg for an adult. The 100 to 300 mcg subcutaneous doses that circulate online come from community posts, not from a trial. Neither is a prescription, because DSIP has no approved human dose.
Key Takeaways
- Every published human DSIP trial found used intravenous dosing, mostly 25 to 30 nmol/kg (about 21 to 25 mcg/kg). FDA's 2026 review found no human data for injection under the skin
- The popular 100 to 300 mcg subcutaneous dose works out to roughly 1.7 to 5 nmol/kg, about 5 to 15 times below the trial dose, by a different route
- In the Reddit file I read, only 3 first-hand reports named a dose (200 mcg, 300 mcg, and a poster who moved from 200 mcg to about 1 mg). That is too thin to call a consensus
- Reconstitution math: a 10 mg vial with 2 mL of water is 5 mg/mL, so 200 mcg is 0.04 mL, or 4 units on a U-100 syringe. Three Reddit posts use three different dilutions, so 100 mcg is 4, 6 or 10 units depending on the post
- DSIP is not FDA-approved. This page is information, not medical advice
For what DSIP is and how the overall evidence looks, see our DSIP peptide guide. This page is the dose-focused companion, and it also covers the side effects and benefits in separate pages so the numbers here stay uncluttered.
DSIP dosage chart: published doses and community doses side by side
The chart below puts the sources on one axis. The first row is the human trial dose converted to micrograms for a 70 kg adult. The other rows are what people say they use. Look at the gap before looking at any single number.
DSIP doses on one axis: human trials vs Reddit
Three things stand out. First, the published trials and the community are not describing the same intervention: one is a slow intravenous infusion given under observation, the other is a self-administered injection or nasal spray. Second, nobody has tested a scaled-down version of the trial dose. Third, the top of the Reddit range, around 1 mg, is a single poster's claim.
| Source type | Route | Dose as stated | Frequency and length |
|---|---|---|---|
| Healthy volunteers, 1981 pilot (6 people) | Slow IV infusion, morning | 25 nmol/kg (about 21 mcg/kg) | Single dose |
| Chronic insomnia trials, 1980s | IV, around bedtime or in the afternoon | 25 to 30 nmol/kg | 3 to 7 consecutive days |
| Alcohol and opiate withdrawal, 1984 (107 patients) | IV | 25 nmol/kg per injection | Up to 6 injections a day for up to 6 days |
| Rat stroke study, 2021 | Intranasal | 120 mcg/kg | 8 days |
| Mouse lifespan study, 2003 | Subcutaneous | About 100 mcg/kg/day of a DSIP and glycine product | 5 days a month, for life |
| Reddit protocol posts (5) | Subcutaneous or nasal | 100 to 300 mcg typical; 500 mcg to 1 mg at the high end | 2 to 12 weeks, often 5 nights on and 2 off |
| Reddit first-hand reports naming a dose (3) | Subcutaneous or nasal | 200 mcg, 300 mcg, 200 mcg moving to about 1 mg | Nightly, or 3 nights on and 2 off |
What the published studies actually dosed
The trial record is old, small and intravenous. The first human study, published in 1981, gave six healthy volunteers 25 nmol/kg by slow intravenous infusion in the morning. The authors reported that total sleep time over the next 130 minutes was 59 percent higher than on placebo (median), that the compound was well tolerated, and that no side effects were observed (Schneider-Helmert 1981, PMID 6895513).
For a molecule of about 849 daltons, 25 nmol/kg is about 21 mcg/kg. A 70 kg adult would receive about 1,490 mcg. The insomnia work that followed used 25 to 30 nmol/kg: one study gave 18 chronic insomniacs six injections at 30 nmol/kg (Schneider-Helmert 1986, PMID 3792404), and a controlled study gave 14 insomniacs seven nights of treatment (Schneider-Helmert 1987, PMID 3622582). The largest study, in 107 inpatients with alcohol or opiate withdrawal, gave repeated intravenous injections (Dick 1984, PMID 6548969). FDA's 2026 briefing document, prepared for the Pharmacy Compounding Advisory Committee, counts 209 people given 25 to 150 nmol/kg intravenously for 1 to 15 days.
How fast it goes in matters. According to FDA's summary of a 1983 multi-study report, the same 25 nmol/kg dose was completely ineffective when infused over 1 minute, and worked best over 4 to 8 minutes. The authors of that report also called slow injection essential (Schneider-Helmert and Schoenenberger 1983, PMID 6689058). The peptide is broken down quickly in blood: FDA cites a plasma half-life of about 8 minutes and in-vitro half-lives of 5 to 10 minutes in human serum. A dose that is injected into fat or sprayed into the nose has an entirely different time course, and no human study has measured it.
The best controlled trial was not encouraging. A double-blind trial in 16 chronic insomniacs gave 25 nmol/kg intravenously on three afternoons. Sleep efficiency was higher and sleep latency shorter than with placebo, but the authors judged the effects weak and in part due to a change in the placebo group, and concluded that short-term treatment was not likely to be of major therapeutic benefit (Bes 1992, PMID 1299794). So even at the doses that were tested, "what dose works" has no clean answer.
Subcutaneous dosing in people has not been studied. FDA states plainly that it found no clinical data on the subcutaneous route, which is the route nearly every dosing chart online assumes. It evaluated a proposed 1 mg/mL injectable and recommended against adding DSIP to the 503A bulk compounding list, citing thin efficacy evidence, safety data that are insufficient, and concerns about characterization, aggregation and immunogenicity. News coverage reports that the advisory committee voted 6 to 7 against as well; I could not confirm the vote on FDA's own page.
Animal doses are not a bridge to human dosing: 120 mcg/kg intranasally in stroke-model rats (Tukhovskaya 2021, PMID 34500605), and about 100 mcg/kg/day of Deltaran, a DSIP and glycine mixture, in monthly five-day courses in mice, as summarized in FDA's briefing document.
What Reddit users report
I read 250 scraped Reddit items from 12 subreddits and 24 threads (2019 to August 2026). Most were noise: a 75-item thread about lithium and Alzheimer's, a 72-item retatrutide thread, vendor sales posts, and app-tracker ads. After removing those, 11 posts or comments from 8 threads in 5 subreddits (r/BiohackingU, r/BodyHackGuide, r/Biohacking, r/insomnia, r/Alzheimers) described first-hand DSIP use. Only 3 of the 11 named a dose. That is too few for any pattern, so treat this section as a handful of anecdotes from a self-selected sample.
The three dose reports: one poster wrote "Ran this at 200mcg right before bed on stressful weeks. Absolute game-changer for nervous system recovery." (a r/BodyHackGuide poster, Aug 2026). Another took 300 mcg nightly inside a stack of ten other compounds and had noticed no side effects yet. The third started at 200 mcg, decided that a dose "closer to 1mg" worked better, took it two to three hours before bed on a 3-nights-on, 2-nights-off schedule, and said the nasal spray works slightly better than the injection (a r/BiohackingU poster, Nov 2025). That third post also carries the author's affiliate link for a DSIP vendor, so weigh it accordingly.
Five more Reddit posts gave protocols without personal experience: a 2019 vendor post (250 mcg, two hours before bed), a cheat sheet (100 to 300 mcg), another cheat sheet (200 mcg nightly for six weeks), a vendor post (100 to 500 mcg), and an AI-compiled guide (start at 100 mcg, with 500 mcg to 1 mg called a typical sweet spot). Two of the five are sales posts. I count them as protocol claims, not user data.
Highest dose each Reddit source named
The timing advice is consistent: 30 to 60 minutes before bed in most posts, up to 2 to 3 hours for two. One commenter described a very different pattern, taking 2 to 3 small doses across the late afternoon, evening and bedtime after a week of no effect from a single bedtime dose, and reported their tracker's deep sleep going from 20 to 30 minutes to about 70. They named no dose, and a wearable is a rough measure. I would not read anything into it beyond "people experiment with timing."
The outlier worth flagging is the 1 mg claim. It is a single post, from an affiliate, with no safety information. It is not evidence that 1 mg is safe or better. For scale: 1 mg is about 17 nmol/kg for a 70 kg adult, within the same order as the trial doses but by a different route and speed.
DSIP reconstitution math
Most DSIP is sold as lyophilized powder in 5 mg or 10 mg vials. The concentration depends only on the vial size and how much bacteriostatic water you add. Divide milligrams by milliliters to get mg/mL, convert to mcg/mL, then divide the dose by the concentration to get milliliters. On a U-100 insulin syringe, 0.01 mL is one unit.
Worked example, 10 mg vial plus 2 mL of water. 10 mg in 2 mL is 5 mg/mL, or 5,000 mcg/mL. A 200 mcg dose is 200 / 5,000 = 0.04 mL, which is 4 units. A 100 mcg dose is 2 units, and 300 mcg is 6 units.
Syringe units for a DSIP dose, by vial and water volume
| 10 mg + 2 mL (5 mg/mL) | 10 mg + 4 mL (2.5 mg/mL) | 5 mg + 3 mL (1.67 mg/mL) | |
|---|---|---|---|
| 100 mcg | 2 units (0.02 mL) | 4 units (0.04 mL) | 6 units (0.06 mL) |
| 200 mcg | 4 units (0.04 mL) | 8 units (0.08 mL) | 12 units (0.12 mL) |
| 300 mcg | 6 units (0.06 mL) | 12 units (0.12 mL) | 18 units (0.18 mL) |
| 1,000 mcg (outlier) | 20 units | 40 units | 60 units |
| Doses per vial at 200 mcg | 50 | 50 | 25 |
Posts do not agree on units. The Reddit posts in my file use three different dilutions: 5 mg in 5 mL (100 mcg is 10 units), 5 mg in 3 mL (6 units), and 5 mg in 2 mL (4 units). All three are arithmetically right for their own water volume. The error happens when someone copies the unit count and not the water volume. Always write down the vial size and the water volume, then calculate from those.
How doses scale by vial size. A 10 mg vial holds 10,000 mcg, so at 200 mcg it provides 50 doses. At 300 mcg it provides 33, and at 100 mcg it provides 100. A 5 mg vial gives half as many. At 5 nights a week and 200 mcg, a 10 mg vial covers about 10 weeks. Doubling the dose halves that, which is why a 1 mg user burns a 10 mg vial in 10 doses.
If you are pricing a vial before you do the math, Ascension Peptides lists DSIP 10 mg at $60.00 as of 2026-10-11 (list price $94.99), which is $6.00 per mg. At 200 mcg that is about $1.20 per dose, so the cost per dose depends far more on your dose than on the vial. Ascension says Kovera Labs tests every batch for HPLC purity, LC-MS identity, net content, endotoxin, sterility and heavy metals, with over 98% of batches passing, and publishes batch COAs. Prices are in USD, shipping is US only and all sales are final. A first-time buyer can use code PEPTIDEDECK for 50% off. Prices move, so check the product page.
Timing, cycle length and what changes the dose
Timing. The trials dosed in the morning (the 1981 pilot), the afternoon (Bes 1992), and one hour before bedtime (the 1983 multi-study report). Community posts say 30 to 60 minutes before bed, and several repeat that DSIP only works during the biological night. That claim is plausible but I did not find a trial that tested it directly; FDA's document notes that natural plasma levels follow the sleep-wake rhythm, which is a different thing.
The graphic below pulls the main numbers from this page into one card.
Cycle length. The longest tested course I found was 15 days of IV dosing (FDA's count), not weeks or months. The 2 to 12 week cycles on Reddit, usually with 1 to 2 weeks off or 5 nights on and 2 off, are community convention. The reasons given, avoiding tolerance and checking whether it still works, are not supported by controlled data either way. A poster on r/Biohacking asked whether three times a week was the maximum before resistance develops, and nobody answered with data.
What changes the dose. The Reddit posts in my file raise two variables: route (two posters say the nasal spray works as well as or better than injection, which I could not verify) and overshooting, since the AI-compiled guide warns that DSIP has a U-shaped curve and that too much can cause agitation or daytime drowsiness. The U-shape does have a source: a 1984 review noted a U-shaped activity curve for both dose and time of infusion in the animal and human data (Graf and Kastin 1984, PMID 6145137). That is a reason not to assume that more is better.
Who should not use this chart at all. Anyone who is pregnant or breastfeeding, anyone taking opioids, benzodiazepines, Z-drugs or other sedatives, anyone with low blood pressure or untreated sleep apnea, and anyone under 18. The reasons are in the side effects page: there are documented cases of low blood pressure after IV injection and no interaction studies.
Frequently Asked Questions
What is the standard DSIP dosage?
There is no standard or approved human dose. The published trials used 25 to 30 nmol/kg intravenously (about 21 to 25 mcg/kg). Community posts commonly cite 100 to 300 mcg under the skin before bed, which is not a tested dose and is not a recommendation.
How many units is 200 mcg of DSIP?
It depends on the water you added. With a 10 mg vial and 2 mL of water (5 mg/mL), 200 mcg is 0.04 mL, or 4 units on a U-100 syringe. With 4 mL of water it is 8 units, and with a 5 mg vial and 3 mL it is 12 units.
How long is a DSIP cycle?
No trial defines one. The human studies ran 1 to 15 days of intravenous dosing. Reddit posts commonly describe 2 to 12 weeks, often 5 nights on and 2 off, but that is convention, not evidence.
Does DSIP work better as a nasal spray?
Some vendors and posters claim so. I found no human data comparing routes. I found one 2001 paper on intranasal DSIP, titled as increasing P300 (a brain-potential measure rather than sleep), but no abstract was available to me, so I could not confirm the dose or species. A 2021 study used a nasal route in stroke-model rats.
Is DSIP FDA-approved?
No. FDA has not approved DSIP for any use, and its 2026 briefing document recommended against adding it to the 503A compounding list. Vials sold online are labeled research use only.
Where to buy research-grade DSIP
If you decide to buy research material despite the thin evidence, Ascension Peptides lists DSIP 10 mg at $60.00 as of 2026-10-11 (list price $94.99). Weigh the dosing gap in this article first, and speak with a clinician if you have a health condition or take medication. See also our guide to buying DSIP.
Medical Disclaimer: DSIP is not approved by the FDA for any condition, and research-grade vials are not intended for human consumption. This article is educational, summarizes published studies and anonymous online reports, and is not medical advice or a recommendation to use or dose any product. Consult a qualified healthcare professional about any health concern.
