This NAD+ dosage chart keeps four routes apart, because the numbers for an injection, a drip and a capsule are not interchangeable. It shows what published human studies gave volunteers, what compounders and clinics list, and what 35 Reddit users say they inject. For the molecule itself, see our NAD+ guide.
Key Takeaways
- The only human dose-ranging data is for oral precursors: NR was tested at 100, 300 and 1,000 mg single doses and up to 2 g/day, NMN at 250 to 1,200 mg/day.
- IV NAD+ has a small pilot (3 micromol/min for 6 hours) and a 2026 clinic review (500 mg/day for 4 days). Subcutaneous and IM NAD+ has no dose-finding trial that I could find.
- Among 35 Reddit accounts, per-injection subcutaneous doses ran 20 to 100 mg, most often 35 to 60 mg or 100 mg, from twice a week to daily.
- The same "10 units" can mean 20 mg or 100 mg depending on how the vial was mixed. Recompute from your own vial.
- NAD+ for injection sold online is a research chemical, not an FDA-approved drug. Nothing here is a recommendation.
The NAD+ dosage chart by route
The table below is the chart most people searching for it want, with the evidence type beside each number. Read the last column first: it tells you how much weight the number can carry.
NAD+ doses by route: trial and clinic data versus user reports
| Dose reported | Schedule or duration | Where it comes from | |
|---|---|---|---|
| Oral NR | 100, 300 and 1,000 mg single doses; 1 g/day; 2 g/day (1 g twice daily) | Single dose; 21 days; 12 weeks | Human trials (PMIDs 27721479, 31412242, 29992272) |
| Oral NMN | 250 mg/day; 300, 600 and 1,200 mg/day | 6 to 12 weeks; 6 weeks | Human trials (PMIDs 35927255, 34238308) |
| IV NAD+ | 3 micromol/min (about 2 mg/min, roughly 700 mg over 6 hours); 500 mg/day | One 6-hour infusion; 4 consecutive days | Small pilot and retrospective clinic review (PMIDs 31572171, 41704678) |
| Subcutaneous / IM NAD+ | 20 to 100 mg per injection among 35 Reddit accounts | Daily to twice weekly | User reports and compounder protocols only; no trial found |
| Sublingual / nasal NAD+ | No human dose data found | Not established | Vendor pages only; 0 first-hand Reddit reports |
Two things stand out. First, the doses that have the most evidence behind them are oral precursor doses in the hundreds of milligrams to grams, not the tens of milligrams people inject. Second, the injectable rows rest on practice, not on dose-finding studies. One competitor page prints a tidy "Starter, Standard, Advanced" ladder for subcutaneous NAD+ and attributes it to community sources; the trials it cites cover IV infusion and oral precursors.
Subcutaneous and intramuscular NAD+ doses
No controlled trial has tested subcutaneous or intramuscular NAD+ for dose, safety or benefit, as far as Europe PMC shows. What exists is compounding-pharmacy protocols and user practice. One US compounding pharmacy's published protocol, for example, lists 30 mg daily under the skin with an optional step to 50 mg Monday to Friday, and 50 mg intramuscularly up to three times weekly. That is a pharmacy's own schedule, not a drug label.
Reddit users sit in a similar range, covered in detail below. The most common pattern is a slow ramp: start around 20 to 25 mg, step up weekly, and settle where side effects stop. The Reddit sample shows a ceiling effect that no chart prints: several people got headaches, jitters or over-stimulation when they went above roughly 70 mg per injection, and went back down.
Intramuscular use is rarer. One user held a prescription for 0.5 mL of a 200 mg/mL vial (100 mg) three times weekly and could not tell whether it was meant for muscle or skin, a question for the prescriber, not Reddit.
IV NAD+ doses
IV is the only injectable route with published human numbers, and they are thin. A 2019 pilot gave volunteers NAD+ at 3 micromol/min for 6 hours. By my arithmetic that is about 2 mg/min, or roughly 700 mg in total. The authors reported that NAD+ was rapidly and completely removed from plasma for at least the first 2 hours, with metabolites consistent with enzymatic breakdown (PMID 31572171). That is pharmacokinetics, not a benefit trial.
A 2026 retrospective review of a commercial clinic compared four consecutive days of 500 mg IV NAD+ against 500 mg IV NR. The NAD+ group reported moderate to severe gastrointestinal symptoms, faster heart rate and chest pressure, and infusions averaged 97 minutes against 37 for NR. Symptoms resolved when the infusions ended (PMID 41704678). The design is retrospective and uncontrolled, so treat it as a tolerability signal.
Clinic menus fill in the rest. One Reddit poster was offered 100 mg or 250 mg at an IV clinic. A compounding pharmacy's protocol describes 250 mg over 2 hours or 500 mg over 4 hours, weekly. The pattern across all of it: the slower the drip, the better people tolerate it, so the infusion rate matters at least as much as the milligram figure. Our side effects page covers the documented reactions.
Sublingual and nasal NAD+ doses
I found no published human dose study for sublingual or nasal NAD+. A few vendor pages list 50 to 100 mg a day under the tongue and 25 to 50 mg a day as a nasal spray, with no trial behind them. In the 384-item Reddit file there were zero first-hand sublingual or nasal reports; one commenter mentioned that a nasal spray can be made, and one recommended a liposomal oral product, both brand-adjacent. Without data on how much reaches the bloodstream, a milligram figure for these routes is a guess, and this page does not offer one.
Oral precursors: NR and NMN trial doses
Here the evidence is real. NR and NMN are not NAD+, they are building blocks the body converts into it, and our NAD+ versus NMN comparison explains the difference. What matters for a dosage chart is what trials gave people.
NR. A pharmacokinetic trial gave single doses of 100, 300 and 1,000 mg and found dose-dependent rises in the blood NAD+ metabolome (PMID 27721479). Twelve older men took 1 g a day for 21 days; muscle NAD+ metabolites rose without a change in mitochondrial bioenergetics (PMID 31412242). Forty obese men took 1,000 mg twice daily for 12 weeks; the regimen looked safe but did not improve insulin sensitivity (PMID 29992272).
NMN. Healthy older men took 250 mg a day for 6 or 12 weeks; blood NAD+ rose and the authors flagged nominal gait and grip changes for validation (PMID 35927255). A 6-week trial in 48 amateur runners used 300, 600 or 1,200 mg a day; the 600 and 1,200 mg groups improved on some aerobic measures relative to placebo, while VO2max and peak power did not differ (PMID 34238308).
Note how far the trial doses are from the "250 to 500 mg" band that some guides print for precursors: researchers went to 1,200 mg of NMN and 2 g of NR. That does not make those amounts advisable. What the raised levels do for health is the separate question our benefits page takes on.
What Reddit users report
I read all 384 items in a scraped set: 23 threads across 14 subreddits. Much was noise, including a long-COVID supplement thread, a protocol list and a vendor ad. After discarding jokes, bots, promotion and second-hand claims, 35 accounts gave a first-hand subcutaneous or IM dose. They came from r/Biohacking (28), r/Peptidesource (5) and r/BodyHackGuide (2). This is a small, self-selected, unverified sample, some of it people on a telehealth or clinic prescription, and it is not advice.
Per-injection subcutaneous NAD+ doses reported on Reddit
The center of the pile is 35 to 60 mg, with a second cluster at exactly 100 mg. By schedule, 11 accounts injected five or more days a week, 14 three to four days, 5 about twice a week or every two to three days, and 5 did not say. Seven described stepping up in stages, for example 25, 50, then 100 mg a week apart. Cycling was uncommon and inconsistent: one described six weeks on and two off, another five days on and two off each week, another had run daily for months without a break.
What people said happened is mixed. Seven described more energy or wakefulness, two described mental clarity, and four said they noticed no clear change, including one who had injected 20 mg Monday to Friday for three months. As that person put it, in a r/Biohacking comment (Aug 2026), "I don’t notice any changes/improvements". Another, in a r/Biohacking thread (Jun 2026), wrote that at 60 mg they "feel like i straight up injected an energy drink". Neither is evidence of what NAD+ does, and the energy reports overlap with people also taking GLP-1 drugs, testosterone or other peptides.
On tolerability, four accounts hit a dose ceiling. One r/Biohacking commenter (Jun 2026) wrote "I’ve up my dose from 50mg to 80mg and I’m having the worst headaches, jitters and I can feel I’m dehydrated". Five reported injection-site burning, one measured a reconstituted pH of 3 to 4, and two said they never felt any burn. Do-it-yourself pH fixes for the sting appeared in the threads, but altering an injectable's pH is a pharmacy job, not a kitchen one.
Treat the outliers as outliers. One commenter claimed to start at 50 to 100 mg daily and move to 125 to 150 mg because NAD+ degrades quickly; I found no support for that. Another reasoned that because some clinics infuse up to 1,000 mg there is little downside to taking more, which does not follow, since infusion is supervised and slow. None of this is evidence of safety.
How to mix NAD+ and read the dose
Most of the chaos in these threads is arithmetic. People ask "how much water do I add" and answer in units, but units only mean something once you know the vial strength and the water volume. The worked example below uses a 500 mg vial.
Mixing a 500 mg NAD+ vial: one worked example
- 1Choose the concentration
500 mg + 2.5 mL bacteriostatic water = 200 mg/mL. - 2Convert dose to volume
Dose divided by concentration. 50 mg / 200 mg/mL = 0.25 mL. - 3Convert volume to units
On a U-100 insulin syringe, mL x 100. 0.25 mL = 25 units. - 4Sanity check
At 200 mg/mL, 10 units = 20 mg and 50 units = 100 mg. Same units, different vial, different dose.
The vial size and water volume change everything, as this table shows for a 50 mg and a 100 mg dose on a 1 mL (100-unit) insulin syringe.
| Vial | Water added | Concentration | 50 mg draw | 100 mg draw |
|---|---|---|---|---|
| 500 mg | 2.5 mL | 200 mg/mL | 0.25 mL (25 units) | 0.5 mL (50 units) |
| 500 mg | 5 mL | 100 mg/mL | 0.5 mL (50 units) | 1 mL (100 units) |
| 1,000 mg | 5 mL | 200 mg/mL | 0.25 mL (25 units) | 0.5 mL (50 units) |
| 1,000 mg | 10 mL | 100 mg/mL | 0.5 mL (50 units) | 1 mL (100 units) |
| 1,000 mg | 2 mL | 500 mg/mL | 0.1 mL (10 units) | 0.2 mL (20 units) |
Three practical points follow. A 1,000 mg vial holds 20 doses of 50 mg or 10 doses of 100 mg, so at 100 mg three times a week it lasts a little over three weeks. A pre-mixed 1,000 mg in 30 mL vial, which one Reddit poster described, is about 33 mg/mL, so a 50 mg dose would be 1.5 mL, more than a 1 mL syringe holds. And one thread showed a protocol of "50 units" with 3, 4 and 5 mL of water per vial and no vial size stated, which cannot be converted to milligrams at all. If the person handing you a protocol does not state vial size and water volume, you do not have a dose.
Storage is the other variable. I found no stability study giving a safe in-use window for a mixed NAD+ vial, so vendor window figures are claims, not data.
Price per dose of research-grade NAD+
Cost shapes behavior here, and several Reddit posters complained that a 500 mg vial lasts five days at 100 mg a day. On 2026-10-11, Ascension Peptides lists NAD+ 1,000 mg at $104.00 (list price $179.99), about $0.10 per mg, or roughly $5.20 for a 50 mg dose and $10.40 for 100 mg. Ascension says Kovera Labs tests every batch for HPLC purity, LC-MS identity, net content, endotoxin, sterility and heavy metals, with more than 98% of batches passing and public batch COAs. Prices are in USD, shipping is US only and all sales are final. New customers can use code PEPTIDEDECK for 50% off. Prices move, so check the product page. For sourcing context see where to buy NAD+ and NAD+ for sale.
Timing, cycles and what changes the dose
Published trials of oral precursors ran 3 to 12 weeks, which is the only controlled duration data on any route. For injections there is no evidence-based cycle length. Users describe everything from continuous use to six weeks on and two off. The injections page covers reported effects and the evidence gap in more depth.
Several factors move a reported dose more than body weight does:
- Route. The same "100" means different things as an injection, a drip or a capsule.
- Concentration. As shown above, the volume drawn changes with how the vial was mixed.
- Tolerance. Reddit users who stepped up found a personal ceiling, often 50 to 80 mg per injection, and dose-limited symptoms were a stated reason to stop climbing.
- Infusion speed. For IV, the rate of the drip is part of the dose.
- Other medicines. Anyone on diabetes, blood pressure or cancer treatment should involve a clinician, because precursors can affect glucose and blood pressure.
Regulatory status and quality
I found no FDA-approved NAD+ injection. In late 2024 the FDA reminded compounders that food-grade NAD+ is not suitable for making sterile injectables, after adverse event reports consistent with endotoxin exposure (FDA notice). Secondary sources also report a 2025 Class I recall of a compounded NAD+ injection for elevated endotoxin, which I could not confirm on an FDA page. Oral NMN is a separate case: the FDA said in September 2025 that NMN is not excluded from the definition of a dietary supplement, per law-firm summaries I did not verify against the letters themselves. Research-grade vials carry none of these oversight layers, which is why batch COAs matter.
Frequently Asked Questions
What is a typical NAD+ dosage?
There is no standard dose. For oral precursors, trials used 250 to 1,200 mg of NMN and 100 mg to 2 g of NR. For injections, 35 Reddit users reported 20 to 100 mg per injection, most often 35 to 60 mg or 100 mg. These are reports, not recommendations.
How much NAD+ should I inject?
No trial answers that, and this page cannot advise it. Reported subcutaneous doses run 20 to 100 mg per injection, usually after a stepped build-up, and several people hit headaches, jitters or nausea above about 70 mg. Talk to a clinician before injecting anything.
How much NAD+ is in an IV drip?
Published data is sparse: a 3 micromol/min infusion over 6 hours (roughly 700 mg by my arithmetic) and a clinic review using 500 mg a day for four days. Clinics also list 100 to 500 mg sessions. Rate of infusion strongly affects tolerability.
How many units is 50 mg of NAD+?
It depends on the vial. At 200 mg/mL, 50 mg is 0.25 mL, or 25 units on a U-100 syringe. At 100 mg/mL it is 50 units. Always divide the dose by your own concentration.
How much NMN or NR should I take?
Trials used NMN at 250 to 1,200 mg a day and NR from single doses of 100 mg to 1 g twice daily. Retail labels often sit lower. What those amounts achieve for health is unclear, and our benefits page grades it.
Where to buy research-grade NAD+
If you decide to buy research material despite the thin dosing evidence, Ascension Peptides lists NAD+ 1,000 mg at $104.00 as of 2026-10-11, with Kovera Labs batch testing and public COAs. Weigh the dosing gaps above first, and speak with a clinician if you have a health condition or take medication.
Medical Disclaimer: NAD+ sold as a research product is not approved by the FDA for any condition and is not intended for human consumption. This article is educational, summarizes published studies, pharmacy protocols and anonymous online reports, and is not medical advice or a recommendation to use any product or dose. Consult a qualified healthcare professional about any health concern.
